Fig. 2

A PAM inhibits HMGB1-induced, but not fMLP-induced cell migration. Mouse 3T3 fibroblasts were subjected to chemotaxis assays in Boyden chambers, 1 nM fr- (fully reduced) HMGB1, or no chemoattractant was added in the lower chamber, together with the indicated concentrations of PAM. Data points with average ± standard deviation (Avg ± SD; n = 4, each point represents a biological replicate) in a representative experiment. Statistics: one-way ANOVA (P = 0.0001), followed by Dunnett’s post-tests. ***P < 0.0004, ****P < 0.0001 relative to no PAM addition. PAM does not inhibit chemotaxis toward fMLP. Data points (n = 4) with Avg ± SD in one representative experiment (each point represents a biological replicate). Migration in the absence or in the presence of the indicated concentration of PAM is not statistically significant (Statistics: one-way ANOVA (P = 0.96). B PAM inhibits chemotaxis toward the HMGB1·CXCL12 heterocomplex. Data points (n = 3) with Avg ± SD in one representative experiment (of three performed in different days). Migration in the presence or absence of PAM is significantly different (P = 0.0002, one-way ANOVA plus Dunnett's post-test; ***P < 0.0004 relative to no PAM addition)