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Fig. 4 | Molecular Medicine

Fig. 4

From: Pamoic acid is an inhibitor of HMGB1·CXCL12 elicited chemotaxis and reduces inflammation in murine models of Pseudomonas aeruginosa pneumonia

Fig. 4

Efficacy of aerosol treatment with PAM (1 mM and 3 mM) in a murine model of P. aeruginosa MDR-RP73 chronic airways infection. C57BL/6NCrlBR male mice (aged 8–10 weeks) received intratracheal inoculation with 5 × 105 CFU of MDR-RP73 strain embedded in agar beads. Treatment started 5 min after infection, with PAM (1 mM and 3 mM) or vehicle administered via aerosol by Penn Century daily for 7 days. Before each administration, mice were weighted, and the percentage change from the initial body weight was averaged for each group of mice (A). Data are presented as mean ± SEM. At day 7 post-infection, mice were sacrificed, BALF was collected and lungs were excised and homogenized. BALF and lung homogenates were plated on tryptic soy agar to determine the bacterial burden (B). Each dot represents total CFU per lung from one mouse, and horizontal lines represent the median values. Total cell (C), neutrophil (D) and macrophage (E) counts were performed on BALF. Neutrophils elastase concentration was evaluated in the supernatants of BALF (F) by ELISA assay. Data are presented as mean ± SEM. Data are pooled from three independent experiments (n = 15–16 mice). Statistical significance is indicated: *P < 0.05

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