Fig. 5

BMSC-exosomes suppress mechanical allodynia in rats by inhibiting the expression of NOTCH2. RT-qPCR (A) and western blotting (B) were used to detect the expression of NOTCH2 in rat SDH; Hind paw PWT (C) and PWL (D) of SNL rats were measured on days 0, 1, 3, 5, 10, 15, and 20 after surgery; (E) TUNEL staining revealed the apoptosis in SDH; (F) ELISA detected the expression levels of TNF-α, IL-1β, and IL-6 in the SDH. Data were shown as mean ± standard deviation. N = 6. ^*P < 0.05, vs. the sh-NC group; ^#P < 0.05, vs. the pcDNA3.1 group; ^&P < 0.05, vs. the pcDNA3.1-NOTCH2 group. BMSC bone marrow mesenchymal stem cell, SNL spinal nerve ligation, SDH spinal dorsal horn, PWT paw withdrawal threshold, PWL paw withdrawal latency