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Fig. 6 | Molecular Medicine

Fig. 6

From: Bone marrow mesenchymal stem cell-derived exosomes shuttling miR-150-5p alleviates mechanical allodynia in rats by targeting NOTCH2 in microglia

Fig. 6

Exosomal miR-150-5p targets NOTCH2 in microglial cells. A StarBase predicted the miRNA-binding sites on NOTCH2; B RT-qPCR detected the expression of NOTCH2-binding miRNAs in BMSC-exosomes; C RT-qPCR detected the expression of the NOTCH2-binding miRNAs in microglial cells that were incubated with BMSC-exosomes or not; RNA pull down assay D and dual-luciferase reporter assay E verified the targeting relationship between miR-150-5p and NOTCH2; RT-qPCR F and western blotting G were used to detect the expression of NOTCH2 in microglial cells overexpressed or underexpressed miR-150-5p. Data were shown as mean ± standard deviation. Cellular experiments were repeated thrice. *P < 0.05, vs. the mimic NC or CM group; #P < 0.05, vs. the inhibitor NC group; &P < 0.05, vs the NC-probe group. BMSC bone marrow mesenchymal stem cell

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Molecular Medicine

ISSN: 1528-3658