Skip to main content
Fig. 1 | Molecular Medicine

Fig. 1

From: Transient Receptor Potential Ankyrin-1-expressing vagus nerve fibers mediate IL-1β induced hypothermia and reflex anti-inflammatory responses

Fig. 1

Neuronal TRPA1 is required for IL-1β-mediated thermoregulation. a Changes in core body temperature in wildtype (B6.129PF) mice in response to i.p. injection of saline (n = 4), 0.5 µg/kg of IL-1β (n = 4), 5 µg/kg of IL-1β (n = 4) and 50 µg/kg of IL-1β (n = 3). The dotted line represents the baseline. Data is averaged over 5-min increments and presented as mean ± SEM (solid line with shaded area). b Area under the curve (AUC) analysis of data in a with respect to a baseline of y = 2. Data presented as individual mouse datapoints with mean ± SEM. One-way ANOVA with Tukey multiple comparison; **p < 0.01, ***p < 0.001. c Changes in core body temperature in TRPA1-Cre/Gq-DREADD mice after i.p. injection (dashed arrow, time 0) of CNO (5 mg/kg) or vehicle (n = 4/group). Two-way RM-ANOVA; ***p < 0.001. d AUC analysis of data in c. Individual mouse datapoints with mean ± SEM presented. Unpaired t-test with Welch’s correction; **p < 0.01. e Changes in core body temperature in wildtype (B6.129PF, n = 4) and TRPA1 KO mice (n = 4) after i.p. administration (dashed arrow, time 0) of IL-1β (5 µg/kg). Data is averaged over 5-min increments and presented as mean ± SEM. 2-way RM-ANOVA; ****p < 0.0001. f AUC analysis of data in e. Data presented as individual mouse datapoints with mean ± SEM. Unpaired t-test with Welch’s correction; ****p < 0.0001. g Changes in core body temperature in wildtype (C57 BL/6, n = 7) and Syn-Cre/TRPA1fl/fl mice (n = 11) after administration (dashed arrow, time 0) of IL-1β (5 µg/kg). Data was averaged over 5-min increments and presented as mean ± SEM. Two-way RM-ANOVA; **p < 0.01. h AUC analysis comparing wildtype and Syn-Cre/TRPA1fl/fl mice with respect to a baseline of y = 2. Data presented as individual mouse datapoints with mean ± SEM. Unpaired t-test with Welch’s correction; **p < 0.01

Back to article page