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Fig. 5 | Molecular Medicine

Fig. 5

From: Transient Receptor Potential Ankyrin-1-expressing vagus nerve fibers mediate IL-1β induced hypothermia and reflex anti-inflammatory responses

Fig. 5

Activation of vagus nerve TRPA1 signaling inhibits TNF during endotoxemia. a TRPA1-Cre/Gq-DREADD mice were injected with either CNO (5 mg/kg) or vehicle i.p. (n = 10/ group) 1 h prior to LPS (0.3 mg/kg, i.p.). For all endotoxemia experiments, serum TNF levels were analyzed at 90 min post-LPS. Individual mouse data points with mean ± SEM are presented. Unpaired t-test with Welch’s correction; *p < 0.05. b Vehicle or 10 µL CNO (0.25 mg/kg) was administered directly to the exposed vagus nerve (n = 7 per group) in TRPA1-Cre/Gq-DREADD mice 2 h prior to LPS (1.0 mg/kg). Individual mouse data points with mean ± SEM are presented. Unpaired t-test with Welch’s correction; *p < 0.05. Optovin was directly applied to the vagus nerve and exposed to sham or 405 nm light stimulation (1000 mA, 10 Hz, with a 10% duty cycle for 5 min) 2 h prior to LPS injection. c Wildtype mice (B6.129PF) receiving sham (n = 9) or light (n = 10) stimulation. Unpaired t-test with Welch’s correction; **p < 0.01. d TRPA1 KO mice receiving sham (n = 9) or light (n = 8) stimulation. Individual mouse data points with mean ± SEM are presented. Unpaired t-test with Welch’s correction; ns: not significant. e Vagotomy was performed either rostral (efferent; n = 12) or caudal (afferent; n = 9) with respect to optovin administration site in wildtype mice (B6.129PF) and subjected to sham or 405 nm light stimulation 2 h prior to endotoxin injection. Individual mouse data points with mean ± SEM are presented. Unpaired t-test with Welch’s correction; *p < 0.05. f Vagus nerve was exposed in α7nAChR KO mice and optovin was directly applied, and subjected to 405 nm light (n = 12) or sham stimulation (n = 10) 2 h prior to LPS injection. Individual mouse data points with mean ± SEM are presented. Unpaired t-test with Welch’s correction; ns: not significant

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