Fig. 2

Accumulation of DNA double-strand breaks (DSBs) and cell cycle arrest in the AECIIs of rats with bronchopulmonary dysplasia (BPD). A Immunofluorescence double staining of γ-H2AX and p180 in rat lung tissue in model and control groups at 14d. (Scale bar = 50 μm, Red fluorescence-labeled γ-H2AX, green fluorescence-labeled p180 represents AECII, blue fluorescence-labeled DAPI represent the nucleus). B The percentage of double-stained cells/p180 positive cells in model and control groups. C Expression level of γ-H2AX in primary AECIIs in model and control groups at different time points (3, 7 and 14 days) detected by western blot. D Cell cycle analyses of primary AECII in model and control groups at 14 days (C = control, M = model). *p < 0.05, **p < 0.01