Fig. 3

Accumulation of DNA double-strand breaks (DSBs) in cultured AECII cells after exposure to hyperoxia. A Cell status detected under the microscope. B DNA damage in cultured AECII cells after exposure to hyperoxia and normoxia at different time points (12, 24 and 48 h) detected by comet assay (Scale bar = 20 μm). C Immunofluorescence staining of γ-H2AX in cultured AECII after exposure to hyperoxia and normoxia at different time points (12, 24 and 48 h). (Scale bar = 10 μm, Red fluorescence-labeled γ-H2AX, blue fluorescence-labeled DAPI represent the nucleus). D Expression levels of γ-H2AX in cultured AECII after exposure to hyperoxia and normoxia at different time points (12, 24 and 48 h) detected by western blot. E Expression levels of p21 in cultured AECII after exposure to hyperoxia and normoxia at 48 h as detected by western blot. F Comet tail length, olive tail moment, and percentage of tail DNA content in cultured AECII cells. G Cell cycle analyses of cultured AECII in model and control groups at 48 h. (C = control, M = model). **p < 0.01; ^##p < 0.01