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Figure 1 | Molecular Medicine

Figure 1

From: Attenuated Hippocampus-Dependent Learning and Memory Decline in Transgenic TgAPPswe Fischer-344 Rats

Figure 1

Biochemical characterization of TgAPPswe rats. A: Western blot analysis of brain protein extracts (at 6 mo of age) from transgenic (Lane 2, TgAPPswe line 17 utilized in this study; Lane 4, another TgAPPswe line but with lower expression) and control nontransgenic (Lanes 3 and 5) inbred Fischer-344 rats. Lane 1 corresponds to APP695 stably transfected CHO cell line extracts that serve as positive control. The hAPP polypeptide is marked (→). B: RNA blot analysis of 12-mo-old TgAPPswe compared with control nontransgenic Fischer rats (non-Tg) revealed 56.8% increase of total APP mRNA levels in TgAPPswe rat samples. Endogenous rat APP (←); transgenic human APPswe (). C and D: Aβ peptide levels in TgAPPswe (n = 6) and control nontransgenic (n = 6) inbred Fischer-344 rats at 12 mo of age. ■, TgAPPswe; □, control nontransgenic Fischer.

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