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Figure 3 | Molecular Medicine

Figure 3

From: Angiotensin II-Mediated Endothelial Dysfunction: Role of Poly(ADP-ribose) Polymerase Activation

Figure 3

AII increases DNA strand breakage in endothelial cells. Treatment of mouse endothelial cells with AII (300 µM) for 24 h increased the number of cells exhibiting open DNA ends from 14% to 38%. Treatment with the AII receptor antagonist losartan, the NADPH oxidase inhibitor apocynin, the NOS inhibitor NG-nitro-L-arginine, but not the xanthine oxidase inhibitor allopurinol, dose-dependently reduced DNA strand breakage following angiotensin treatment. Results are expressed as mean ± SEM of the % of cells from 3 separate experiments with a total of at least 800 nuclei counted for each treatment. Statistical significance was determined using Student t test where P < 0.05 was considered significant; *P < 0.05, **P < 0.01 compared with untreated cells and †P < 0.05 compared with AII treatment alone.

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