Figure 1
From: Deficiency of Neuropilin 2 Suppresses VEGF-Induced Retinal Neovascularization
Measurement of Npn2 mRNA during retinal development and in a model of ischemic retinopathy. C57BL/6 mice were euthanized at several time points after birth with or without exposure to 75% oxygen between P7 and P12, and total retinal RNA was isolated. Real-time quantitative reverse transcription-polymerase chain reaction was performed to measure the level of Npn2 and cyclophilin mRNA. Cyclophilin A mRNA is generally modulated very little and serves as a reference for normalization. Data are expressed as the number of copies of Npn2 transcripts per 105 copies of cyclophilin A transcripts, and each bar represents the mean (±SEM) calculated from the retinas of 4 mice. Npn2 expression increased throughout retinal development and reached a stable steady-state level by P15. Exposure to hyperoxia resulted in a significant increase in Npn2 expression (P < 0.01 for difference between P7 room air and P8 hyperoxia), which decreased by the 5th day of oxygen exposure and then briefly increased 1 d after removal from oxygen.