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Figure 4 | Molecular Medicine

Figure 4

From: Deficiency of Neuropilin 2 Suppresses VEGF-Induced Retinal Neovascularization

Figure 4

Mice deficient in Npn2 develop less ischemia-induced retinal NV. Littermate npn2−/− (n = 7), npn2+/− (n = 5), and npn2+/+ mice (n = 5) were exposed to 75% oxygen between P7 and P12 and euthanized on P17. Retinal frozen sections were stained with Griffonia simplicifolia lectin and visualized with DAB, so that vascular cells appear brown. Representative sections from an npn2−/− mouse (A, D) show occasional small areas of retinal neovascularization extending above the internal limiting membrane. In contrast, representative sections from littermate npn2+/− (B, E, arrows) or npn2+/+ mice (C, F, arrows) show many large fronds of retinal neovascularization extending above the internal limiting membrane. Measurement of the area of retinal neovascularization above the internal-limiting membrane on serial sections as described in Materials and Methods demonstrated that npn2−/− mice had significantly less retinal neovascularization than npn2+/− or npn2+/+ (ANOVA with Bonferroni correction for multiple comparisons).

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