Figure 7

Effect of in vivo treatment with EGCG on activation of AP-1 and phosphorylation of c-Jun in rats subjected to myocardial ischemia and reperfusion. EGCG-treated rats received a bolus dose of EGCG 10 mg/kg at the end of the ischemia period followed by 10 mg/kg/h intravenous infusion for the entire period of reperfusion. A: Representative autoradiograph of electrophoretic mobility shift assay for AP-1 in vehicle-treated rats and EGCG-treated rats at 0 (sham value), 30, 60, and 120 min after reperfusion. The autoradiograph is representative of 3 separate experiments performed at different times. B: Western blot analysis demonstrating the effect of vehicle or EGCG on treatment phosphorylation of c-Jun at 0 (sham value), 30, 60, and 120 min after reperfusion. Western blot analysis is representative of separate experiments performed at different times. Fold increase was calculated compared with respective sham value (time 0) set to 1.0. Results are representative of 3 separate time-course experiments. *P < 0.05 compared with respective sham value (time 0); #P < 0.05 compared with vehicle-treated rats subjected to myocardial ischemia and reperfusion.