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Figure 5 | Molecular Medicine

Figure 5

From: Reduced Peripheral PGE2 Biosynthesis in Plasmodium falciparum Malaria Occurs through Hemozoin-Induced Suppression of Blood Mononuclear Cell Cyclooxygenase-2 Gene Expression via an Interleukin-10-Independent Mechanism

Figure 5

Effect of hemozoin on COX-2 mRNA expression. COX-2 mRNA was quantified for 4 individuals by real time RT-PCR. PBMCs (1 × 106 cells/mL) were stimulated with media alone, LPS (100 ng/mL) and IFN-γ (200 U/mL), and LPS and IFN-γ in the presence of hemozoin (10, 1.0, and 0.1 µg/mL) or β-hematin (10, 1.0, and 0.1 γg/mL). Cells were collected at 48 h for COX-2 mRNA determination. Values are the mean ± SEM, n = 4. Statistical significance was determined by the Mann-Whitney U test. *P < 0.01 compared with control (media alone), **P < 0.01 compared with stimulated conditions.

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Molecular Medicine

ISSN: 1528-3658