Figure 2
From: Intracellular Evaluation of ER Targeting Elucidates a Mild Form of Inherited Coagulation Deficiency
Expression levels of FVII variants. (A) Activity of FVII variants (expressed as % of WtFVII) in medium collected at 6, 12, and 24 h after transient transfection (5 independent experiments). (B-C) Left panels. Protein levels of FVII variants in medium and organelles from transiently (B, 5 independent experiments) or stably (C, 3 independent clones) transfected cells in 24 h. Protein levels in C are reported in log scale to better detail low levels of variants. Results are reported as average ± standard deviation. (B-C) Right panel. Detection by Western blotting of the β-actin (∼42 kDa) and PDI (∼55 kDa) proteins as markers of the cytosolic and organelle fractions. Molecular weights are given in kDa.