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Figure 4 | Molecular Medicine

Figure 4

From: Selective Inhibition of the Human tie-1 Promoter with Triplex-Forming Oligonucleotides Targeted to Ets Binding Sites

Figure 4

Electrophoretic mobility shift assays showing triplex DNA formation at the E-1 tandem Ets binding motif sequence of the human tie-1 promoter. Radiolabeled oligonucleotide encoding the C/T-rich strand of the tie-1 promoter E-1 site ([32P]-E-1 oligo) was annealed to the cold complementary G/A strand (see Figure 1), and incubated with increasing concentrations of phosphodiester (A) and phosphodiester-phosphorothioate-linked (B) cold T1-ap (Figure 1), in binding buffer containing 10 mM MgCl and 150 mM KCl.

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