Figure 5

Selective inhibition of tie-1 promoter activity with the T1-ap triplex-forming oligonucleotide. Tie-1 or SV40 promoter-driven luciferase reporter constructs (ptie-1luc and pSV40luc, respectively) were incubated with phosphodiester (20 µM) (A) or partial phosphorothioate (PSO)-modified T1-ap (B), T2-ap and control oligonucleotides (T1-P, T2-P, and T1-(C/T)-P; see Figure 1) in binding buffer for 18 h and transfected into bovine aortic endothelial cells. After 72 h, reporter activity was determined by dual luciferase assay and normalized to the co-transfected pRL-CMV control plasmid. Results are means (± 1 SD, bars) of three to five experiments (n = 6–10) expressed as a percentage of the T1-P-treated control reporter plasmid.