Real-Time PCR and Linkage Studies to Identify Carriers Presenting HPRT Deleted Gene

Table 1 Features of microsatellites used in the linkage analysis

Microsatellite	Primers	Average heterozygosity	Distance from HPRT gene	Range of allelic size
D×S907	forward CAGTGTTTCAGTCTTCACCAGGAC	0	∼134 kb	127–140 bp
D×S907	reverse GCCCATGAATTGAAATGAATGCC
D×S8074	forward TTATAAATTAGCCAGAGGTGTTG	0.60	∼480 kb	221–231 bp
D×S8074	reverse CTAGGTGTGTCTGTAAAGGTAGG
D×S8041	forward GCAAGACTCCGTCTCAAATAATAAC	0.36	∼87 kb	144–164 bp
D×S8041	reverse TTCCTGCTACCTGCAATTCC

Average heterozygosity was calculated on 50 controls; distance between microsatellites and the middle of HPRT gene and size range of fragments were calculated by UCSC Genome Browser.

ISSN: 1528-3658