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Table 3 Real-time PCR results.

From: Real-Time PCR and Linkage Studies to Identify Carriers Presenting HPRT Deleted Gene

Samples

n

Mean

Range of value

SD of 2−ΔΔCt t value

Negative male control

6

1.01

0.94–1.08

0.0449

Negative female control

6

1.07

1.00–1.14

0.0602

Positive control

3

0

—

—

Carrier

3

0.49

0.48–0.51

0.0173

  1. Average value of the 2−ΔΔCt is the result of each performed experiment. Results fluctuate in a small range of values. We show standard deviation as calculated for each sample in each experiment.
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Molecular Medicine

ISSN: 1528-3658