Effect of ITF2357 on IL-6 production. Freshly cultured PBMCs were preincubated with ITF2357 for 1 h and then stimulated with the combination of IL-18 (10 ng/mL) and IL-12 (1 ng/mL) as in Figure 8. Open bars indicate PBMCs at a concentration of 2.5 million cells/mL, and solid bars represent PBMCs at 5 million cells/mL. Mean ± SEM of IL-6 measured in the supernatant of triplicate wells after 24 h are shown for 2 donors. *P < 0.05; **P < 0.01 compared with no ITF2357.