Effect ITF2357 on apoptosis and necrosis in PBMCs. (A) PBMCs in RPMI were incubated with increasing concentrations of ITF2357 for 1 h followed by the addition of LPS (10 ng/mL) to appropriate wells. After 24 h at 37° C, the percentage of annexin-V- and PI-positive PBMCs was determined. The data are the mean ± SEM of 5 separate experiments/donors. (B) FACS analysis of PI and annexin V staining in control PBMCs. (C) PI and annexin V staining in PBMCs incubated with 100 nM ITF2357. (D) PI and annexin V staining in PBMCs incubated with 1000 nM ITF2357. The data in B, C, and D are from 1 representative experiment.