Suppression of IL-18/IL-12-induced IFNγ by ITF2357. Freshly cultured PBMCs were pretreated with increasing concentrations of ITF2357 (shown under horizontal axis) for 1 h as described in Figure 7. The cells were stimulated with the combination of IL-18 (10 ng/mL) plus IL-12 (1 ng/mL) (left panel). Matching cultures were similarly stimulated in the presence of IL-1Ra (10 µg/mL, middle panel) or TNF-bp (1 µg/mL, right panel). IFNγ was measured in the supernatants after 24 hours. Data are mean ± SEM of IFNγ in ng/mL of 4 donors. *P < 0.05; **P < 0.01 compared with no ITF2357.