Figure 9

Stimulus-dependent suppression of IFNγ by ITF2357. (A) LPS-induced IFNγ from resting PBMCs stimulated with LPS (10 ng/mL). Resting PBMCs were preincubated with ITF2357 as indicated for 1 h at 37° C before the addition of LPS. Mean ± SEM of IFNγ in pg/mL of 4 donors. (B) After preincubation with ITF2357, the cells from the same donors were stimulated with anti-CD3 (500 ng/mL). After 24 h, IFNγ was measured in the supernatants. Mean ± SEM of IFNγ in pg/mL of 3 donors are shown.