Figure 2

Effect of treatment with anti-HMGB1 antibody on HS/R-induced gut mucosal permeability (A) and bacterial translocation to MLN (B). Ileal mucosal permeability and bacterial translocation were assessed 24 h after HS/R (or the sham procedure). Mice in the sham group (n = 6) were subjected to anesthesia and vascular cannulation but not hemorrhage. Mice in the IgG (n = 6) and aHMGB1 (n = 8) groups were shocked by withdrawal of blood over 10 min until MAP decreased to 25 mmHg. MAP was maintained at 25 to 30 mmHg for 2 h, and then the mice were resuscitated by infusing all of the remaining shed blood plus a volume of Ringer’s lactate solution equal to twice the total volume of shed blood. Mice in the IgG group were treated with a single intraperitoneal dose of rabbit IgG (600 µg in 500µL) immediately after the resuscitation procedure. Mice in the aHMGBl group were similarly treated, except these animals received a dose of a polyclonal rabbit neutralizing aHMGBl antibody. Mice in the sham group were treated with PBS (500µL intraperitoneally). Results are means ± SEM. *P < 0.05 vs. sham; †P< 0.05 vs. IgG.