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Figure 11 | Molecular Medicine

Figure 11

From: Predifferentiated Embryonic Stem Cells Prevent Chronic Pain Behaviors and Restore Sensory Function Following Spinal Cord Injury in Mice

Figure 11

ES cells in vitro. Live culture pictures (A, C, E). (A) Free-floating embryoid bodies. (C) Differentiation induction, neural progenitors sprouting from embryoid body. (E) Neural network of differentiated cells. Immunocytochemical staining of cultured ES cells (B, D, F). (B) GFP-positive embryoid body viewed under fluorescence microscopy. Mitotic, histone H3-positive cells were visualized with Texas red. (D) Neural network. Staining against synapsin. (F) Double immunostaining of RA differentiated ES cells. Positive staining against Map-2 conjugated with FITC for green fluorescence indicates neuronal phenotype; LIM-2 conjugated with Texas red for red fluorescence allows further characterization of highly specialized dorsal interneurons.

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Molecular Medicine

ISSN: 1528-3658