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Table 1 Genes with detectable expression in healthy blood donor samples, together with statistical summaries of ΔCT distribution, expression fold changes corresponding to 2 standard deviations of ΔCT distribution, and P values for normality tests.

From: Limited Dynamic Range of Immune Response Gene Expression Observed in Healthy Blood Donors Using RT-PCR

HUGO Designation Gene Name and Aliases n Mean Median SD Fold Change ±2 SD Shapiro-Wilk Anderson-Darling
ADAM17 A Disintegrin and Metalloproteinase Domain 17 129 18.56 18.55 0.63 2.39 0.7512 0.5395
APAF1 Apoptotic Protease Activating Factor 1 131 16.46 16.48 0.54 2.13 2.1E-05 0.0150
C1QA Complement Component 1, Q Subcomponent, Alpha Polypeptide 128 20.25 20.21 0.92 3.57 0.0939 0.0879
CD14 CD14 Antigen 129 13.92 14.01 0.63 2.41 1.1E-05 8.0E-07
CD19 CD19 Antigen 131 18.19 18.09 0.78 2.94 1.4E-05 1.1E-07
CD4 CD4 Antigen 131 14.80 14.84 0.49 1.98 0.0064 3.8E-04
CD86 CD86 Anitigen; B7-2 Protein 128 17.64 17.68 0.51 2.04 3.1E-05 6.6E-04
CD8A CD8 Antigen, Alpha Polypeptide, p32 130 15.74 15.72 0.67 2.54 0.0653 0.8402
CXCL1 Chemokine (C-X-C Motif) Ligand 1 (GRO-1) 131 20.01 20.00 0.67 2.53 0.1150 0.1522
CYBB Cytochrome B-245 Beta Polypeptide 130 13.98 14.02 0.57 2.21 0.0058 0.0542
DPP4 Dipeptidylpeptidase IV (CD26) 131 18.33 18.35 0.61 2.34 0.1253 0.0602
EGR1 Early Growth Response 1 130 20.42 20.49 0.65 2.47 0.0074 0.0013
ELA2 Elastase 2, Neutrophil 126 19.90 19.78 1.29 5.95 2.1E-04 1.4E-04
GCLC Glutamate-Cysteine Ligase, Catalytic Subunit 128 18.86 18.90 0.64 2.41 5.6E-07 2.9E-05
HMGB1 High-Mobility Group Box 1 130 16.28 16.25 0.69 2.59 0.0055 0.0524
HMOX1 Heme Oxygenase (Decycling) 1 131 16.45 16.50 0.67 2.53 0.0028 0.0045
HSPA1A Heat Shock Protein 1A, 70 kDa 129 13.83 13.88 0.80 3.01 3.7E-08 1.2E-06
ICAM1 Intercellular Adhesion Molecule 1 131 17.68 17.71 0.55 2.15 0.0969 0.0514
IFI16 Interferon γ-Inducible Protein 16 130 16.75 16.72 0.84 3.20 0.0441 0.1004
IL10 Interleukin 10 75 22.87 22.94 0.75 2.81 9.0E-04 0.0070
IL15 Interleukin 15 129 21.45 21.45 0.70 2.65 0.0051 0.0275
IL18 Interleukin 18 (Interferon γ-Inducing Factor) 130 20.05 20.05 0.54 2.11 0.0517 0.0625
IL18BP IL-18 Binding Protein 131 16.74 16.72 0.44 1.84 0.2787 0.6132
IL1B Interleukin 1β 130 16.67 16.67 0.79 2.99 0.0011 0.0200
IL1R1 Interleukin 1 Receptor, Type I 125 21.08 21.06 0.98 3.90 0.5969 0.9508
IL1RN Interleukin 1 Receptor Antagonist 129 16.88 16.91 0.67 2.54 0.1494 0.1755
IL8 Interleukin 8 97 21.01 20.86 1.46 7.53 0.0321 0.1185
LTA Lymphotoxin α 114 20.05 19.99 0.65 2.45 3.4E-04 0.0014
MMP9 Matrix Metalloproteinase 9 129 15.91 16.01 1.16 4.97 3.8E-05 1.9E-06
MNDA Myeloid Cell Nuclear Differentiation Antigen 130 12.54 12.51 0.64 2.44 0.1193 0.1563
MPO Myeloperoxidase 131 21.20 21.22 0.77 2.92 0.7944 0.7479
MYC V-myc Avian Myelocytomatosis Viral Oncogene Homolog 130 17.23 17.22 0.63 2.38 0.0685 0.0705
NFKB1 Nuclear Factor of κ Light Polypeptide Gene Enhancer in B Cells 1 (p105) 131 17.38 17.41 0.57 2.20 0.0178 0.0076
PLA2G7 Phospholipase A2, Group VII 126 19.36 19.36 0.69 2.60 0.1485 0.5492
PLAUR Plasminogen Activator, Urokinase Receptor 131 15.12 15.15 0.58 2.25 0.0275 0.0190
PTGS2 Prostaglandin-Endoperoxide Synthase 2 (COX-2) 126 16.72 16.75 0.61 2.33 0.0505 0.0309
PTPRC Protein Tyrosine Phosphatase Receptor, 127 Type C (CD45) 11.91 11.96 0.52 2.07 0.0410 0.0165  
SERPINA1 Serine (or Cysteine) Proteinase Inhibitor, Clade A, Member 1 (Alpha 1 Anti-Trypsin) 131 13.26 13.27 0.66 2.50 0.0100 0.0092
SERPINE1 Serine (or Cysteine) Proteinase Inhibitor, Clade E (Ovalbumin), Member 1 (Plasminogen Activator Inhibitor Type 1) 101 22.38 22.44 0.89 3.43 0.0014 0.0015
SERPING1 Serine (or Cysteine) Proteinase Inhibitor, Clade G (C1 Inhibitor), Member 1 (Angioedema, Hereditary) 130 19.20 19.29 1.20 5.25 3.2E-04 6.0E-05
TGFB1 Transforming Growth Factor β 1 130 13.14 13.16 0.44 1.83 0.0115 0.0141
TIMP1 Tissue Inhibitor of Matrix Metalloproteinase 1 131 15.02 15.09 0.57 2.19 1.1E-05 4.2E-06
TLR2 Toll-Like Receptor 2 130 16.07 16.13 0.63 2.38 0.0058 0.0015
TNF Tumor Necrosis Factor 124 20.67 20.55 0.98 3.90 1.1E-05 3.4E-04
TNFSF5 Tumor Necrosis Factor (Ligand) Superfamily, Member 5 (CD40 Ligand) 131 17.69 17.67 0.63 2.38 1.5E-14 1.5E-10
TNFSF6 Tumor Necrosis Factor (Ligand) Superfamily, Member 6 (Fas Ligand) 126 20.41 20.35 0.74 2.80 4.7E-04 0.0021
  1. n is the number of samples having detectable expression for the gene in at least 3 of 4 replicate RT-PCR reactions. Mean and SD are estimated by maximum likelihood for genes where any replicates fall below the detection threshold (CT > 37).
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Molecular Medicine

ISSN: 1528-3658