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Table 1 Genes with detectable expression in healthy blood donor samples, together with statistical summaries of ΔCT distribution, expression fold changes corresponding to 2 standard deviations of ΔCT distribution, and P values for normality tests.

From: Limited Dynamic Range of Immune Response Gene Expression Observed in Healthy Blood Donors Using RT-PCR

HUGO Designation

Gene Name and Aliases

n

Mean

Median

SD

Fold Change ±2 SD

Shapiro-Wilk

Anderson-Darling

ADAM17

A Disintegrin and Metalloproteinase Domain 17

129

18.56

18.55

0.63

2.39

0.7512

0.5395

APAF1

Apoptotic Protease Activating Factor 1

131

16.46

16.48

0.54

2.13

2.1E-05

0.0150

C1QA

Complement Component 1, Q Subcomponent, Alpha Polypeptide

128

20.25

20.21

0.92

3.57

0.0939

0.0879

CD14

CD14 Antigen

129

13.92

14.01

0.63

2.41

1.1E-05

8.0E-07

CD19

CD19 Antigen

131

18.19

18.09

0.78

2.94

1.4E-05

1.1E-07

CD4

CD4 Antigen

131

14.80

14.84

0.49

1.98

0.0064

3.8E-04

CD86

CD86 Anitigen; B7-2 Protein

128

17.64

17.68

0.51

2.04

3.1E-05

6.6E-04

CD8A

CD8 Antigen, Alpha Polypeptide, p32

130

15.74

15.72

0.67

2.54

0.0653

0.8402

CXCL1

Chemokine (C-X-C Motif) Ligand 1 (GRO-1)

131

20.01

20.00

0.67

2.53

0.1150

0.1522

CYBB

Cytochrome B-245 Beta Polypeptide

130

13.98

14.02

0.57

2.21

0.0058

0.0542

DPP4

Dipeptidylpeptidase IV (CD26)

131

18.33

18.35

0.61

2.34

0.1253

0.0602

EGR1

Early Growth Response 1

130

20.42

20.49

0.65

2.47

0.0074

0.0013

ELA2

Elastase 2, Neutrophil

126

19.90

19.78

1.29

5.95

2.1E-04

1.4E-04

GCLC

Glutamate-Cysteine Ligase, Catalytic Subunit

128

18.86

18.90

0.64

2.41

5.6E-07

2.9E-05

HMGB1

High-Mobility Group Box 1

130

16.28

16.25

0.69

2.59

0.0055

0.0524

HMOX1

Heme Oxygenase (Decycling) 1

131

16.45

16.50

0.67

2.53

0.0028

0.0045

HSPA1A

Heat Shock Protein 1A, 70 kDa

129

13.83

13.88

0.80

3.01

3.7E-08

1.2E-06

ICAM1

Intercellular Adhesion Molecule 1

131

17.68

17.71

0.55

2.15

0.0969

0.0514

IFI16

Interferon γ-Inducible Protein 16

130

16.75

16.72

0.84

3.20

0.0441

0.1004

IL10

Interleukin 10

75

22.87

22.94

0.75

2.81

9.0E-04

0.0070

IL15

Interleukin 15

129

21.45

21.45

0.70

2.65

0.0051

0.0275

IL18

Interleukin 18 (Interferon γ-Inducing Factor)

130

20.05

20.05

0.54

2.11

0.0517

0.0625

IL18BP

IL-18 Binding Protein

131

16.74

16.72

0.44

1.84

0.2787

0.6132

IL1B

Interleukin 1β

130

16.67

16.67

0.79

2.99

0.0011

0.0200

IL1R1

Interleukin 1 Receptor, Type I

125

21.08

21.06

0.98

3.90

0.5969

0.9508

IL1RN

Interleukin 1 Receptor Antagonist

129

16.88

16.91

0.67

2.54

0.1494

0.1755

IL8

Interleukin 8

97

21.01

20.86

1.46

7.53

0.0321

0.1185

LTA

Lymphotoxin α

114

20.05

19.99

0.65

2.45

3.4E-04

0.0014

MMP9

Matrix Metalloproteinase 9

129

15.91

16.01

1.16

4.97

3.8E-05

1.9E-06

MNDA

Myeloid Cell Nuclear Differentiation Antigen

130

12.54

12.51

0.64

2.44

0.1193

0.1563

MPO

Myeloperoxidase

131

21.20

21.22

0.77

2.92

0.7944

0.7479

MYC

V-myc Avian Myelocytomatosis Viral Oncogene Homolog

130

17.23

17.22

0.63

2.38

0.0685

0.0705

NFKB1

Nuclear Factor of κ Light Polypeptide Gene Enhancer in B Cells 1 (p105)

131

17.38

17.41

0.57

2.20

0.0178

0.0076

PLA2G7

Phospholipase A2, Group VII

126

19.36

19.36

0.69

2.60

0.1485

0.5492

PLAUR

Plasminogen Activator, Urokinase Receptor

131

15.12

15.15

0.58

2.25

0.0275

0.0190

PTGS2

Prostaglandin-Endoperoxide Synthase 2 (COX-2)

126

16.72

16.75

0.61

2.33

0.0505

0.0309

PTPRC

Protein Tyrosine Phosphatase Receptor, 127 Type C (CD45)

11.91

11.96

0.52

2.07

0.0410

0.0165

 

SERPINA1

Serine (or Cysteine) Proteinase Inhibitor, Clade A, Member 1 (Alpha 1 Anti-Trypsin)

131

13.26

13.27

0.66

2.50

0.0100

0.0092

SERPINE1

Serine (or Cysteine) Proteinase Inhibitor, Clade E (Ovalbumin), Member 1 (Plasminogen Activator Inhibitor Type 1)

101

22.38

22.44

0.89

3.43

0.0014

0.0015

SERPING1

Serine (or Cysteine) Proteinase Inhibitor, Clade G (C1 Inhibitor), Member 1 (Angioedema, Hereditary)

130

19.20

19.29

1.20

5.25

3.2E-04

6.0E-05

TGFB1

Transforming Growth Factor β 1

130

13.14

13.16

0.44

1.83

0.0115

0.0141

TIMP1

Tissue Inhibitor of Matrix Metalloproteinase 1

131

15.02

15.09

0.57

2.19

1.1E-05

4.2E-06

TLR2

Toll-Like Receptor 2

130

16.07

16.13

0.63

2.38

0.0058

0.0015

TNF

Tumor Necrosis Factor

124

20.67

20.55

0.98

3.90

1.1E-05

3.4E-04

TNFSF5

Tumor Necrosis Factor (Ligand) Superfamily, Member 5 (CD40 Ligand)

131

17.69

17.67

0.63

2.38

1.5E-14

1.5E-10

TNFSF6

Tumor Necrosis Factor (Ligand) Superfamily, Member 6 (Fas Ligand)

126

20.41

20.35

0.74

2.80

4.7E-04

0.0021

  1. n is the number of samples having detectable expression for the gene in at least 3 of 4 replicate RT-PCR reactions. Mean and SD are estimated by maximum likelihood for genes where any replicates fall below the detection threshold (CT > 37).