Limited Dynamic Range of Immune Response Gene Expression Observed in Healthy Blood Donors Using RT-PCR

McLoughlin, Kevin; Turteltaub, Ken; Bankaitis-Davis, Danute; Gerren, Richard; Siconolfi, Lisa; Storm, Kathleen; Cheronis, John; Trollinger, David; Macejak, Dennis; Tryon, Victor; Bevilacqua, Michael

doi:10.2119/2006-00018.McLoughlin

Molecular Medicine

Table 2 Sex, age, and ethnicity (fixed effect) contributions to intersubject variation for 43 genes, in decreasing order of percentage of variance explained (equation 3).

From: Limited Dynamic Range of Immune Response Gene Expression Observed in Healthy Blood Donors Using RT-PCR

Gene	Percent of Variance Explained by Sex, Age, and Ethnicity	P Values for Effect							Fold Change Corresponding to Effect			Fold Change Corresponding to Age Difference (69 vs. 23 years)
Gene	Percent of Variance Explained by Sex, Age, and Ethnicity	Sex	Ethnicity	Sex + Ethnicity	Age	Sex + Age	Ethnicity + Age	Sex + Ethnicity + Age	WM vs. WF	HF vs. WF	HM vs. WF	WF	WM	HF	HM
NFKB1	27.90	0.0022	0.7083	0.1098	0.3335	0.0553	0.1896	0.2572	1.35	−1.04	−1.03	−1.29	−2.48	1.32	1.43
MPO	27.74	0.0128	0.0005	0.0116	0.2260	0.3535	0.8682	0.0909	1.42	1.82	1.31	1.57	1.00	1.43	4.48
IL18	27.46	0.0220	0.6228	0.9333	0.0119	0.0468	0.9150	0.1023	1.25	1.06	1.30	1.97	−1.02	1.88	2.77
MYC	26.91	0.0180	0.0132	0.7298	0.5240	0.1344	0.9658	0.1843	1.31	−1.42	−1.17	−1.21	−2.19	−1.24	1.23
TGFB1	25.88	0.0121	0.0726	0.5024	0.5696	0.0809	0.3451	0.1967	1.22	−1.18	−1.07	−1.13	−1.84	1.21	1.47
TNFSF6	23.90	0.0008	0.0344	0.0123	0.1039	0.0892	0.8499	0.4898	1.62	1.39	1.17	1.78	−1.28	1.61	1.32
LTA	23.87	0.0179	0.8223	0.0131	0.4992	0.1109	0.9253	0.4470	1.32	−1.03	−1.33	−1.22	−2.32	−1.17	−1.27
ELA2	23.65	0.3536	0.0013	0.0998	0.0262	0.2949	0.1695	0.0889	1.24	2.53	1.50	4.21	1.78	1.09	6.94
CD86	21.03	0.0289	0.2781	0.4782	0.1418	0.1509	0.3672	0.3403	1.24	1.13	1.23	1.47	−1.11	2.12	2.42
CD14	20.75	0.0066	0.9157	0.1150	0.1484	0.4972	0.0631	0.6869	1.42	−1.02	−1.05	−1.65	−2.24	1.64	1.71
C1QA	19.11	0.9650	0.2648	0.7856	0.0458	0.1489	0.0286	0.0015	1.01	1.25	1.15	2.51	1.07	−1.89	9.27
GCLC	19.04	0.1281	0.0510	0.4470	0.1961	0.5864	0.4943	0.1936	1.19	1.31	1.33	1.51	1.21	2.10	−1.66
HSPA1A	18.91	0.0208	0.3329	0.8448	0.2111	0.5560	0.1404	0.3747	1.37	−1.17	1.12	−1.58	−2.09	1.46	2.47
TNF	18.22	0.0489	0.6451	0.2638	0.6774	0.8716	0.0660	0.4877	1.44	−1.11	−1.14	−1.23	−1.11	3.34	1.59
HMGB1	17.72	0.0630	0.0047	0.1891	0.2164	0.3968	0.6109	0.9320	1.24	1.50	1.39	1.48	1.05	1.16	−1.31
CYBB	17.34	0.0223	0.9489	0.3272	0.9352	0.2462	0.1217	0.5252	1.31	−1.01	1.05	1.03	−1.56	2.17	2.22
SERPINA1	17.27	0.0730	0.2471	0.9965	0.2167	0.5933	0.0938	0.5365	1.28	−1.21	1.06	−1.59	−2.06	1.66	2.28
MMP9	16.14	0.1858	0.1057	0.3289	0.1730	0.6537	0.5372	0.3199	1.36	−1.57	1.34	−2.39	−3.46	−1.31	2.56
CXCL1	15.91	0.1965	0.1305	0.5228	0.3722	0.4229	0.1961	0.4267	1.18	−1.26	1.09	−1.36	−1.94	1.46	2.02
EGR1	15.53	0.0263	0.3387	0.4572	0.2550	0.0909	0.6418	0.2217	1.33	−1.15	−1.04	1.48	−1.44	1.16	1.54
IL15	15.44	0.4922	0.0501	0.0862	0.3364	0.9222	0.3302	0.2744	1.10	1.37	−1.03	1.42	1.36	2.45	−1.15
DPP4	15.00	0.2114	0.4354	0.4450	0.1223	0.6509	0.1781	0.5079	1.15	−1.11	−1.14	−1.63	−1.95	1.18	−1.70
CD4	14.86	0.0816	0.4197	0.3359	0.9445	0.0935	0.4285	0.4223	1.19	−1.10	−1.11	1.02	−1.77	1.42	1.34
HMOX1	14.81	0.0587	0.8654	0.6194	0.2167	0.0801	0.9441	0.0954	1.27	−1.03	1.10	1.53	−1.44	1.59	3.05
PLA2G7	14.20	0.0865	0.9282	0.7051	0.4431	0.8269	0.0575	0.6794	1.27	1.02	1.17	−1.34	−1.49	2.31	3.07
PLAUR	13.46	0.2046	0.4947	0.9590	0.4582	0.2254	0.6593	0.1645	1.17	−1.10	1.07	−1.28	−2.15	−1.02	1.83
TIMP1	13.33	0.0658	0.5106	0.2000	0.4669	0.1399	0.8392	0.3714	1.23	−1.09	−1.17	1.25	−1.44	1.37	1.51
CD8A	13.23	0.0412	0.1604	0.8832	0.2939	0.7299	0.4394	0.8534	1.30	1.24	1.56	−1.44	−1.24	1.05	1.44
ADAM17	12.71	0.1919	0.8616	0.6441	0.1633	0.2687	0.7874	0.7724	1.15	1.02	1.29	1.49	−1.01	1.68	1.37
PTPRC	12.65	0.0279	0.7490	0.7072	0.5853	0.4100	0.2291	0.5759	1.24	1.04	1.20	−1.15	−1.53	1.41	1.53
PTGS2	12.55	0.0630	0.9846	0.6788	0.0812	0.9289	0.1552	0.9724	1.28	−1.00	1.15	−1.87	−1.79	1.17	1.18
IL1RN	12.41	0.3848	0.0842	0.1495	0.1451	0.6460	0.1061	0.8547	−1.13	−1.33	−1.03	−1.73	−1.38	1.48	2.18
ICAM1	11.99	0.2755	0.2793	0.7173	0.4375	0.4882	0.2839	0.3328	1.13	−1.16	1.06	−1.27	−1.68	1.31	2.10
APAF1	11.91	0.0852	0.8732	0.8466	0.8508	0.4636	0.0957	0.6159	1.20	−1.02	1.13	−1.05	−1.38	1.96	1.06
MNDA	11.64	0.0662	0.9441	0.3044	0.5092	0.6863	0.3526	0.3483	1.24	−1.01	−1.03	−1.23	−1.46	1.28	2.29
IL18BP	10.94	0.1220	0.0913	0.1308	0.6556	0.3362	0.4393	0.4857	1.14	1.18	1.06	1.11	−1.20	1.45	1.62
SERPING1	10.70	0.4339	0.2313	0.9868	0.5224	0.9825	0.7008	0.2993	−1.21	−1.41	−1.69	−1.52	−1.49	−1.03	5.36
IL1R1	10.49	0.1039	0.7632	0.9654	0.7602	0.3975	0.2078	0.9550	1.34	−1.07	1.28	−1.16	−2.00	2.25	1.40
IL1B	10.09	0.6746	0.3584	0.4113	0.3254	0.7338	0.1561	0.9919	−1.07	−1.19	1.01	−1.52	−1.84	1.68	1.40
TLR2	9.82	0.2787	0.6278	0.5153	0.7197	0.1332	0.2841	0.8863	1.15	1.08	1.06	1.14	−1.77	2.05	1.16
TNFSF5	9.48	0.3867	0.3648	0.1478	0.0599	0.4390	0.3545	0.6951	1.12	1.16	−1.12	−2.01	−1.39	−1.19	−1.18
IFI16	6.46	0.5860	0.6442	0.5085	0.2437	0.8924	0.4439	0.9634	1.09	1.09	−1.02	−1.62	−1.74	1.00	−1.02
CD19	5.24	0.1827	0.4304	0.1925	0.3513	0.1437	0.4404	0.5098	1.23	1.16	−1.03	1.48	−1.51	−1.11	−1.25

Values were computed only for white and Hispanic subjects for whom sex and age were recorded (n = 68). Unadjusted Pvalues are shown for each effect, including interaction terms, and underlined (together with corresponding fold changes) when < 0.05. Fold changes for sex and ethnicity effects are computed by raising 2 to the power of the corresponding ΔC_T effect terms; for age effects, they are computed by multiplying the corresponding slope effect by the range of ages in the sample (69 − 23) and then exponentiating. HF indicates Hispanic female; HM, Hispanic male; WF, white female; WM, white male.

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