Effect of VEGF165 on keratinocyte proliferation. MTT-based assays were performed to determine the effects of VEGF alone or in combination with VEGFR-2 neutralizing antibody for 48-h treatment. Data represent the mean values of optical density measured in 6 wells for each treatment in a representative experiment. This experiment was repeated at least 3 times with similar results. **P ≤ 0.01; ***P < 0.001, significant differences between VEGF-treated and untreated samples. #P < 0.05, significant difference between VEGF-treated cells and cells treated with both VEGF and VEGFR-2 neutralizing antibody.