Immunolocalization and Expression of Vascular Endothelial Growth Factor Receptors (VEGFRs) and Neuropilins (NRPs) on Keratinocytes in Human Epidermis

Table 1 PCR primers used in this study.

Gene	GenBank accession no.	Primer sequence	Location	Product length (bp)
VEGFR-1	NM_002019	5′-ATGGCTCCCGAATCTATCTTTGAC-3′	3445-3468	655
		5′-GCCCCGACTCCTTACTTTTACTGG-3′	4099-4076
VEGFR-2	NM_002253	5′-CTGGCGGCACGAAATATCCTCTTA-3′	3388-3411	614
		5′-GGCCGGCTCTTTCGCTTACTGTTC-3′	4001-3978
VEGFR-3	NM_182925	5′-AGGCCGGCCCACGCAGACATC-3′	2424-2444	346
		5′-TGCACGCCCCGAGGAGGTTGA C-3′	2769-2748
NRP-1	NM_001024629	5′-AGCCCCTCCTCCTGTTGTGTCTTC-3′	461-484	441
		5′-GCTATCGCGCTGTCGGTGTAAAAA-3′	901-878
NRP-2	NM_201264	5′-CAGCCGGCCCCCAGCACAT-3′	29-47	436
		5′-TTCCCGAAAGCCAAGCGTAACAAT-3′	464-441
CD31	BC051822	5′-CTTCGCGGATGTCAGCACCAC-3′	384-404	419
		5′-CCTCAACGGGGAATTCCAGTATCA-3′	802-779
GAPDH	NM_002046	5′-TGAAGGTCGGAGTCAACGG-3′	113-131	223
		5′-TGGAAGATGGTGATGGGAT-3′	335-317

ISSN: 1528-3658