Table 1 PCR primers used in this study.
Gene | GenBank accession no. | Primer sequence | Location | Product length (bp) |
|---|---|---|---|---|
VEGFR-1 | NM_002019 | 5′-ATGGCTCCCGAATCTATCTTTGAC-3′ | 3445-3468 | 655 |
|  |  | 5′-GCCCCGACTCCTTACTTTTACTGG-3′ | 4099-4076 |  |
VEGFR-2 | NM_002253 | 5′-CTGGCGGCACGAAATATCCTCTTA-3′ | 3388-3411 | 614 |
|  |  | 5′-GGCCGGCTCTTTCGCTTACTGTTC-3′ | 4001-3978 |  |
VEGFR-3 | NM_182925 | 5′-AGGCCGGCCCACGCAGACATC-3′ | 2424-2444 | 346 |
|  |  | 5′-TGCACGCCCCGAGGAGGTTGA C-3′ | 2769-2748 |  |
NRP-1 | NM_001024629 | 5′-AGCCCCTCCTCCTGTTGTGTCTTC-3′ | 461-484 | 441 |
|  |  | 5′-GCTATCGCGCTGTCGGTGTAAAAA-3′ | 901-878 |  |
NRP-2 | NM_201264 | 5′-CAGCCGGCCCCCAGCACAT-3′ | 29-47 | 436 |
|  |  | 5′-TTCCCGAAAGCCAAGCGTAACAAT-3′ | 464-441 |  |
CD31 | BC051822 | 5′-CTTCGCGGATGTCAGCACCAC-3′ | 384-404 | 419 |
|  |  | 5′-CCTCAACGGGGAATTCCAGTATCA-3′ | 802-779 |  |
GAPDH | NM_002046 | 5′-TGAAGGTCGGAGTCAACGG-3′ | 113-131 | 223 |
|  |  | 5′-TGGAAGATGGTGATGGGAT-3′ | 335-317 |  |