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Table 1 PCR primers used in this study.

From: Immunolocalization and Expression of Vascular Endothelial Growth Factor Receptors (VEGFRs) and Neuropilins (NRPs) on Keratinocytes in Human Epidermis

Gene GenBank accession no. Primer sequence Location Product length (bp)
VEGFR-1 NM_002019 5′-ATGGCTCCCGAATCTATCTTTGAC-3′ 3445-3468 655
   5′-GCCCCGACTCCTTACTTTTACTGG-3′ 4099-4076  
VEGFR-2 NM_002253 5′-CTGGCGGCACGAAATATCCTCTTA-3′ 3388-3411 614
   5′-GGCCGGCTCTTTCGCTTACTGTTC-3′ 4001-3978  
VEGFR-3 NM_182925 5′-AGGCCGGCCCACGCAGACATC-3′ 2424-2444 346
   5′-TGCACGCCCCGAGGAGGTTGA C-3′ 2769-2748  
NRP-1 NM_001024629 5′-AGCCCCTCCTCCTGTTGTGTCTTC-3′ 461-484 441
   5′-GCTATCGCGCTGTCGGTGTAAAAA-3′ 901-878  
NRP-2 NM_201264 5′-CAGCCGGCCCCCAGCACAT-3′ 29-47 436
   5′-TTCCCGAAAGCCAAGCGTAACAAT-3′ 464-441  
CD31 BC051822 5′-CTTCGCGGATGTCAGCACCAC-3′ 384-404 419
   5′-CCTCAACGGGGAATTCCAGTATCA-3′ 802-779  
GAPDH NM_002046 5′-TGAAGGTCGGAGTCAACGG-3′ 113-131 223
   5′-TGGAAGATGGTGATGGGAT-3′ 335-317