Retinoic Acid, GABA-ergic, and TGF-β Signaling Systems Are Involved in Human Cleft Palate Fibroblast Phenotype

Table 1 Sequences of oligonucleotide primers and PCR conditions

Gene name	Size, bp	5′ Oligonucleotide	3′ Oligonucleotide	GenBank accession no.	Annealing temperature, °C	Cycles^a, n
β-Actin	351	(522)-CACACTGTGCCCATCTACGAGG-(543)	(872)-AGTTTCGTGGATGCCACAGGA-(852)	X00351	60	23
TGF-β3	298	(262)-GCACTTGCAAAGGGC TCTG-(280)	(559)-GGCATAGTATTCCGACTCGG-(540)	NM_0003239	58	28
TGFBRI	277	(780)-CCTCTAGAGAAGAACGTTCGT-(800)	(1056)-GGCTTTCCTTGGGTACCAACAA-(1035)	NM_004612	58	26
TGFBR2	387	(1092)-GGTCGCTTTGCTGAGGTCTA-(2011)	(1478)-CTTGAGGTCCCTGTGCACGAT-(1458)	NM_003242	58	23
TGFBR3	363	(1621)-CCTGTCATTCCCAGCATACAACT-(1643)	(1983)-ATCACCTGACTCCAGATCTTCATA-(1960)	XM_001924	58	25
RARA	298	(491)-GCATCATCAACAAGGTGACC-(510)	(788)-CTGAACTTGTCCCAGAGGTCA-(768)	NM_000964	56	22
GABRB3	299	(243)-CCTTTGTGAAGGAGACGGT-(261)	(541)-CACTCCATGCACAAATGAC-(523)	NM_000814	54	22

^aThe number of cycles was adjusted to be in the exponential phase of the amplification of each product.

ISSN: 1528-3658