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Figure 2 | Molecular Medicine

Figure 2

From: Differential Reactions of Microglia to Brain Metastasis of Lung Cancer

Figure 2

Double immunolabeling for (A) lectin (for microglia and endothelium, green) and iNOS (red); (B) lectin (green) and TNF-α (red); (C) GFAP (for astrocytes, green) and iNOS (red); and (D) GFAP (green) and TNF-α (red). The DAPI (purple) stains the nuclei in all micrographs. A dense population of lectin-positive cells (microglia or endothelia, green) is observed in A and B. However, most lectin-positive cells are not colocalized with either iNOS (A) or TNF-α (B). The GFAP-positive cells (green) are not labeled with iNOS (C), but a few GFAP-positive astrocytes express TNF-α (D, yellow cells indicated by arrows). Some GFAP-negative cells are found to express TNF-α at the left upper-view field of D. Bar = 50 µm.

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