Figure 6

MTT assays of the cellular effect of TNF-α on viability of the lung cancer cells. Recombinant mouse TNF-α at 0.388, 0.97, 1.94, 2.91, and 3.88 ng/mL was cocultured with the lung cancer cells for 12, 24, 36, and 48 h. The viability of the lung cancer cells was determined using MTT assays. No significant decrease in viability can be noted, while increases in viability can be observed in at 24 and 48 h after treatment with higher concentrations of TNF-α. The data are presented as means and standard deviations. Statistically significant differences (P < 0.05) are found between the following pairs of groups: 1) At 24 h, 0.338 ng/mL, equal to 10% LPS-BVCM treatment group vs. 3.88 ng/mL, 100% LPS-BVCM treatment group; 2) At 48 h, 0.338 ng/mL, 10% group vs. 2.91 ng/mL, 75% or 3.88 ng/mL,