Figure 3
From: Activation of the Poly(ADP-Ribose) Polymerase Pathway in Human Heart Failure
Detection of poly(ADP-ribosyl)ation. Human ventricular tissue homogenates (D, donor; F, failing) were loaded onto 5% to 20% gradient SDS-polyacrylamide gels (20 µg of protein in each lane) and probed with a poly-ADP-ribose-specific antibody (1:10,000; Calbiochem). According to the manufacturer’s data sheet, this antibody recognizes poly-ADP-ribosylated proteins specifically and serum albumin nonspecifically (indicated). The most prominent nitrotyrosine-specific band (indicated) was further evaluated for purposes of densitometry. Mean values and SEM are shown in the bar graph (donor, n = 5; failing, n = 8; P = 0.005).