Figure 3

Viral particle entry and transduction efficiency after endosomolytic agent treatment. (A) To address entry of viral particles following transduction, we labeled the particles with YOYO-1 before transduction. In some cases, cells were treated with chloroquine before transduction. After removal of uninternalized and surface-bound particles, fluorescence intensity was assessed using flow cytometry at 4 h after transduction. The results are expressed as mean relative fluorescence intensity ± SD. (B) HSVECs were transduced with MMLV, MMLV plus polybrene (PB), virosomes and immunovirosomes (carrying anti-CD71 antibodies). Percentage of transduced cells was analyzed using flow cytometry. Results comparing treatment with or without chloroquine were compared using the paired t test (shown with + chloroquine data) with * indicates P < 0.001 and # indicates P < 0.05. These are representative of three independent experiments. Results were analyzed with paired t test. *P < 0.001; #P< 0.05.