Figure 4

Early kinetics of gene expression. HSVECs that had been stimulated with 80 ng/mL TNF-α, 80 ng/mL IFN-γ, and 80 ng/mL IL-1β) for 8 h were treated with amphotropic MMLV-EGFP in combination with polybrene or amphotropic MMLV-EGFP containing virosomes or immunovirosomes (made with anti-CD71 mAb). After transduction/transfection, the cells were cultured for 24 h. Flow cytometric analysis was performed at 30, 60, 180, 360, 720, and 1440 min to determine the percentage of EGFP-positive cells (transfection efficiency) (A) and the mean fluorescence intensity of cell (B). All results are expressed as the mean (% transfection efficiency) ± SD of triplicates (A) and the mean fluorescence intensity (B).