Figure 5

Kinetics of gene expression after gene transfer. HSVECs that had been stimulated with 80 ng/mL TNF-α, 80 ng/mL IFN-γ, and 80 ng/mL IL-1β) for 8 h were treated with amphotropic MMLV-EGFP in combination with polybrene or amphotropic MMLV-EGFP containing virosomes or immunovirosomes (made with anti-CD71 mAb) (upper panel). Alternatively, the cells were transfected with pCMV-EGFP plasmid DNA using liposomes or immunoliposomes containing anti-CD71 mAb (lower panel). After gene transfer, the cells were cultured for 25 days. Flow cytometric analysis was performed every 3 days to determine the percentage of EGFP positive cells. All results are expressed as the mean (% of EGFP positive cells) ± SD of triplicates.