Figure 1
From: Passive Immunization to Outer Membrane Proteins MLP and PAL Does Not Protect Mice from Sepsis
Immunoblot analysis of rabbit anti-MLP and anti-PAL. Purified MLP, PAL, boiled whole bacteria of MLP and PAL deletion mutant strains and their wild-type parent strains, and the E. coli strains used in the animal models were used as antigens. Positions of MLP and PAL bands are indicated by arrows on the left. Molecular mass markers (kDa) are at right. (A). Anti-MLP IgG. Lane 1, purified MLP (50 ng); Lane 2, JE5506 E. coli K12; Lane 3, JE5505, the corresponding E. coli K-12 MLP deletion mutant; Lane 4, E. coli O4; Lane 5, E. coli O18K+. (B). Anti-PAL IgG. Lane 1, purified PAL (50 ng); Lane 2, E. coli K-12 1292; Lane 3, JC7752, the corresponding E. coli K-12 PAL deletion mutant; Lane 4, E. coli O4; Lane 5, E. coli O18K+.