Figure 3
From: Phosphorothioate Oligonucleotides Reduce PrPSc Levels and Prion Infectivity in Cultured Cells
N-CAM, tubulin, and PrP levels in ScN2a and N2a cells after exposure to PS-DNA. ScN2a and N2a cells incubated for 2 weeks with various concentrations of scrambled 22-mer PS-DNA indicate decreased levels of PrPC and PrPSc but no change in the level of the other proteins. Similar results were seen for shorter incubation times (data not shown). Cell lysates without PK digestion (-PK) were probed with PrP antibody (D13), and antibodies against N-CAM and tubulin on the same immunoblot. This can be accomplished because N-CAM, tubulin, and PrP have different molecular weights (140 kDa, 49.5 kDa, and 19.6 to 37.4 kDa, respectively). C, control with no PS-DNA.