Figure 4

Effect of PRL-3 siRNA on migration and invasion of B16-BL6 melanoma cells. In both assays, B16-BL6 cells were untreated or transfected with Luc-siRNA or PRL-3 siRNA (1 and 2 µg) for 24 h. Each cell suspension (100 µL, 1 × 10⁶ cells/mL) was plated into the upper wells of Transwell inserts containing 8-µm pore polycarbonate membranes precoated with fibronectin (10 µg/mL) on the undersurface or also with Matrigel on the upper surface in the invasion assay. Cells were allowed to migrate for 24 h at 37°C, then those on the upper wells were removed gently and those that migrated to the undersurface were fixed and stained. (A) Quantitative analysis of the number of the cells that migrated to the lower side of the membrane in the migration assay. (B) Histogram of the invasion assay performed with B16-BL6 cells and the relative transfectants. Data are mean ± SEM of three independent experiments. **P < 0.01 vs. Luc siRNA control.