Figure 3

Survivin expression in TSC2 cells. A: Hematoxylin and eosin staining of TSC2^−/− A⁺ cells (a), TSC2^+/− R⁺ cells (b), and A549 cells (c); immunocytochemical staining revealed that the R⁺ (b′) and A549 cells (c′) were rich in survivin, whereas the A⁺ cells were negative (a′). B: Western blot analysis of survivin expression in A⁺, R⁺, and A549 cells (upper panel); as an internal control of gel loading, the membrane also was decorated with β-actin antibody (lower panel). Western blotting did not detect survivin in the TSC2^−/− A⁺ cells, but showed its abundance in TSC2^+/− R⁺ and A549 cells.