Figure 5

A. Effect of various formulations of DAS on the expression of p53wt in mouse skin tumors. Skin/tumor lysates were prepared as described in Materials and Methods. Lysates were resolved by electrophoresis and analyzed for p53wt using anti-p53wt antibodies. To quantify equal loading, membranes were re-probed with β-actin antibody. The intensity of the bands was quantitated using Image Analysis software on an Image Gel Documentation System. Lane 1, untreated; lane 2, DMBA + Acetone; lane 3, DMBA + Cream; lane 4, DMBA + Sham-Lip; lane 5, DMBA + Sham-pH-Lip; lane 6, DMBA + Free DAS; lane 7, DMBA + Lip-DAS; lane 8, DMBA + pH-Lip-DAS and lane 9, Cream base only with no DMBA. B. Effect of various formulations of DAS on the expression of p53mut in mouse skin tumors. Skin/tumor lysates were prepared as described in Materials and Methods and analyzed for p53mut expression by Western blot analysis using antibody to p53mut. To quantify equal loading, membranes were reprobed with β-actin antibody. The intensity of the bands was quantitated using Image Analysis software on an Image Gel Documentation System. Lane 1, untreated; lane 2, DMBA + Acetone; lane 3, DMBA + Cream; lane 4, DMBA + Sham-Lip; lane 5, DMBA + Sham-pH-Lip; lane 6, DMBA + Free DAS; lane 7, DMBA + Lip-DAS; lane 8, DMBA + pH-Lip-DAS and lane 9, Cream base only with no DMBA.