Reparixin decreases short-term (24 h) PMN infiltrate in the ischemic area. Representative histochemistry (400 ×) of the ischemic cortex 24 h after transient (A–B) or permanent (C–D) MCAO with vehicle (A, C) or reparixin (B, D). PMN are stained in red. No PMN was seen in sham-operated rats (not shown). Quantification of PMN by MPO activity is shown in panel E where data are expressed as ΔA/min/mg protein (difference between the ipsilateral and the contralateral hemisphere) and are the mean ± SE (number of rats in each group is indicated in parentheses). * P < 0.05, ** P < 0.01 vs. respective MCAO + saline by Student t-test.