Figure 6

The intensity of eNOS immunolabelling evaluated by scoring system in sham-operated animals, vehicle-treated I/R animals, and I/R animals treated with EPO five minutes before ischemia and at the onset of reperfusion. Grades of score: mild (1), moderate (2), strong (3), and very strong (4). Data are mean ± S.E.M. (n = 3–5 per group). *P < 0.05 vs sham; **P < 0.05 vs vehicle-treated I/R animals; ***P < 0.01 vs vehicle-treated I/R animals (one-way ANOVA followed by Tukey’s post-test). Note that there is a significant decrease in EPO groups compared with the I/R group. Sham; intestinal I/R was not induced, I/R; intestinal I/R, EPO + I/R; rHuEPO administered five minutes before ischemia, I + EPO + R; rHuEPO administered onset of reperfusion.