Table 1 Oligonucleotides Used for Real Time RT-PCR
MRNA | Sequence (5′-3′) | Product (bp) | GenBank Accession No. |
|---|---|---|---|
betaglycan (sense) | CCTGTCATTCCCAGCATACAAT | 363 | XM001924 |
betaglycan (reverse) | ATCACCTGACTCCAGATCTTCATA | ||
Decorin (sense) | GGCCACTATCATCCTCCTT | 270 | NM133503 |
Decorin (reverse) | TTGTTGTTTTGCAGGTCTAGC | ||
syndecan (sense) | TCTGACAACTTCTCCGGCTC | 210 | NM001006946 |
syndecan (reverse) | CCACTTCTGGCAGGACTACA | ||
biglycan (sense) | GCTCCGACCTGGGTCTGCTGAA | 306 | NM001711 |
biglycan (reverse) | ACTCCCTTGGGCACCTTGC | ||
MMP-1 (sense) | CTCATGCTTTTCAACCAGGC | 370 | X05231 |
MMP-1 (reverse) | GTGCGCATGTAGAATCTGTC | ||
FGF2 (sense) | AGTGTGTGCTAACCGTTACC | 214 | NM002006 |
FGF2 (reverse) | AAGTATAGCTTTCTGCCCAGG | ||
β-actin (sense) | ACCTTCTACAATGAGCTGCG | 197 | NM001101 |
b-actin (reverse) | TCCATCACGATGCCAGTGGTA |