Expression of ZnT-1 is followed by attenuation of Zn2+ influx and toxicity. A. HEK-293 cells were co-transfected with ZnT-1 and LTCC plasmids, or with LTCC only. Cells were loaded with Fura-2 (5 εM), which is also a highly sensitive Zn2+ dye, and imaged while superfusing with a high K+ Ringer’s solution (50mM) thereby opening the LTCC in the presence of Zn2+ (200 εM). B. Neurons were transfected with a ZnT-1 siRNA construct or control siRNA. Zn2+ (200 εM) was applied to a neuronal culture in the presence of high K+ Ringer’s (ten minutes). Cell death was determined 24 h later using LDH.