Cellular location of three human LIV-1 family members in MCF-7 cells. MCF-7 cells expressing recombinant HKE4, LIV-1, or ZIP14 were imaged using a mouse anti-V5 antibody (Invitrogen) conjugated to Alexa Fluor 488 (green) and assembled onto slides using Vectorshield with DAPI (Vector Laboratories). All cells were incubated with Texas red phalloidin to stain F-Actin filaments red. Coverslips were viewed on a Leica RPE automatic microscope using a 63x oil immersion lens. The fluorescent superimposed images were acquired using a multiple bandpass filter set appropriate for DAPI, fluorescein, and Texas Red as well as bright field for differential interference contrast imaging.