Figure 1
From: Differential Gene Expression after Zinc Supplementation and Deprivation in Human Leukocyte Subsets
Intracellular labile zinc following incubation with zinc or TPEN. Cells were cultured in the presence of 50 µM ZnSO4 or 2.5 µM TPEN for 40 h. For the last 30 min, cells were loaded with the zinc-specific fluorescent dye FluoZin-3, and the resulting zinc-specific fluorescence was measured by flow cytometry and transformed into labile zinc concentrations as described in the methods section. Data shown are means from n = 4 independent experiments ± SE.