Gel shift analysis using nuclear extracts from PBMC proteins of different subjects with 32P-labeled oligonucleotide probe harboring an NF-kB consensus sequence. Very low NF-kB binding activity was observed in the healthy pregnant women (lane a), a moderate level of binding activity was observed in disease control (pregnant women with AVH) (lanes b and c), while a very high binding activity was observed in the hepatitis virus infected FHF patients, both nonpregnant (lane d) and pregnant (lane e and f). The positions of the specific retarded bands are indicated. The squares mark the unspecific complexes. The extent of binding activity as revealed by densitometry analysis is indicated below each lane.