Figure 2

(A) EMSA using PBMC proteins from the FHF patients, with a ³²P-labeled oligonucleotide harboring an NF-kB consensus sequence. Binding specificity was evidenced by pre-incubation with a 100-fold molar addition of the homologous unlabeled oligonucleotide (lane b) in comparison with competition experiments using a heterologous consensus sequence of the Oct-1 transcription factor (lane c). The squares mark the unspecific complexes. (B) EMSA carried out with labeled oligos encompassing a consensus sequence of Oct-1 transcription factor showing uniform binding activity in PBMC proteins from different subjects. Lane a, normal healthy pregnant women as controls (N); lane b, hepatitis virus infected FHF pregnant patients (F); lane c, PBMC protein of patients who recovered back to normalcy (R). The positions of the specific retarded bands are indicated.