Electromobility supershift analysis using nuclear extracts from PBMC of (A) healthy pregnant women without hepatitis virus infection, (B, C) pregnant FHF patients infected with different hepatitis viruses, and (D, E) FHF patients after their recovery to normalcy, with 32P-labeled oligonucleotides harboring an NF-kB consensus sequence showing differential binding activity of NF-kB components. PBMC nuclear extracts were incubated with specific antibodies (Abs) recognizing different members of the NF-kB family. Lane a, without Ab; lane b, addition of p50 Ab; lane c, addition of p65 Ab. The position of the NF-kB specific complex and the additionally retarded complexes is indicated with arrows. The squares mark the unspecific complexes. The dark circles mark the free oligos. The extent of supershifting as revealed by densitometry analysis are indicated below each lane and was derived in comparison to the non-shifted NF-kB complex in lane a.